Table 1 Top 25 variants ranked by enrichment rate from the naive to the PS4 library.

From: Bridging non-overlapping reads illuminates high-order epistasis between distal protein sites in a GPCR

Variant

Enrichment rate

Fold increase in enrichment rate over WT

SLNIG

791

2635

TSWIH

787

2624

TTYLH

635

2117

TGRIA

517

1722

TTTLG

436

1454

TLTLN

371

1235

TGHIA

347

1157

TSWLHa

336

1121

TGKLA

310

1034

SLTLQ

244

813

TTWLS

231

772

TGKLS

224

747

TTWLHa

193

642

TLYLN

178

592

TAWLHa

149

497

TQYLGb

148

494

TCWIH

142

474

TLMVH

122

406

TLWIH

121

405

MLNIG

118

393

TGRLG

114

379

SLNMG

109

365

TCWLHa

100

332

TFWIN

96

319

TGRLS

91

302

  1. aSingle-residue mutant, bProximal double-residue mutant.