Fig. 7: Evolutionary history of the YBR196C-A locus.
From: De novo emergence of adaptive membrane proteins from thymine-rich genomic sequences
a YBR196C-A emerged in an ancestral non-genic sequence with high TM propensity. Results of ancestral reconstruction software are summarized along the Saccharomyces genus phylogenetic tree. Branch lengths are not meaningful. Theoretical translations of extant and reconstructed sequences are shown, with ORF boundaries indicated by a green M and a red star. For ORF-less sequences, frames displayed were chosen for illustration purposes. Except for the intergenic ancestor, the translation of the sequence that aligns to the start codon of YBR196C-A is shown, in the relevant frame, and until the first stop codon is reached. TM propensity as predicted by Phobius is shown in pink. b–d Predicted 3D structures for the translation products of YBR_Initial (b), YBR_Intermediate (c) and YBR196C-A (d). Model 1 predictions by Robetta are shown. YBR_Initial is predicted to encode an all-β strand protein. YBR_Intermediate is predicted to encode a protein with β-strands and an α-helix. YBR196C-A is predicted to encode a protein with a long α-helix and no β-strands.
