Fig. 4: RR468 phosphorylation and dephosphorylation at different pHs.
From: Revisiting the pH-gated conformational switch on the activities of HisKA-family histidine kinases
a Time course of RR468 phosphorylation upon incubation with acetyl-phosphate (AcP) as phosphodonor at different pHs. b Phosphatase activity of HK853 over phosphorylated RR468 in the absence and presence of ADP. Gels correspond to a representative experiment and quantification of three independent experiments are plotted. Error bars represent SD. Source data are provided as a Source Data file.
