Fig. 6: Autophosphorylation of EnvZ and phosphotransfer to OmpR.
From: Revisiting the pH-gated conformational switch on the activities of HisKA-family histidine kinases
a Time course of EnvZ autophosphorylation with [γ-32P] ATP at different pHs. b Stability of phosphorylated EnvZ at acidic (5) and basic (8) pH. Half-life plots for each pH is shown. c Phosphotransfer of phosphorylated EnvZ to OmpR at acidic (right) and basic (left) pH in the absence and presence of ADP. Gels correspond to a representative experiment and quantification of three independent experiments are plotted. Error bars represent SD. Source data are provided as a Source Data file.
