Fig. 4: pti-DIMs promote genome-stabilising clustering of Rad52 droplets. | Nature Communications

Fig. 4: pti-DIMs promote genome-stabilising clustering of Rad52 droplets.

From: DNA repair by Rad52 liquid droplets

Fig. 4

a–f Live-cell microscopy shows that the pti-DIM-compromising tub3Δ (a, b) lowers droplet velocity (c), increases droplet numbers (d) and decreases droplet size (e), while western blotting shows Rad53 hyper-phosphorylation (f). kar3∆ served as control. Quantifications represent the mean ± s.d.; *P ≤ 0.0004 in χ2 (b, d) and Mann–Whitney U (c, e) tests. n = 3 independent experiments, 120 cells total (b). n = 127 (WT) and 80 droplets (tub3∆) over 6 independent experiments (c). n = 11, 440 cells (WT), n = 3, 120 cells each (tub3∆, kar3∆) (d). n = 123 (WT), 169 (tub3∆), and 83 (kar3∆) (e). Scale bars, 1 µm. Source data are provided as a Source Data file.

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