Fig. 4: Complementation of in vivo CHIL function by heterologous CHILs in an A. thaliana chil mutant. | Nature Communications

Fig. 4: Complementation of in vivo CHIL function by heterologous CHILs in an A. thaliana chil mutant.

From: A conserved strategy of chalcone isomerase-like protein to rectify promiscuous chalcone synthase specificity

Fig. 4

a RT-PCR analyses of the wild type (Col-0), the chil-3 mutant, and the complemented chil-3 mutants of A. thaliana for expression of the CHIL gene. b Seed phenotypes of Col-0, the chil-3 mutant, and the complemented chil-3 mutants. c Contents of soluble proanthocyanidins in mature seeds of Col-0 (black bar), the CHS mutant (tt4, green bar), the CHIL mutants (chil-1 and chil-3; white bars), and the complemented chil-3 mutants of A. thaliana with the CHIL gene (red bars), the CHI gene (light-blue bars) or the empty vector with 35SGUS (white bar). d Contents of insoluble proanthocyanidins in mature seeds of the A. thaliana lines shown in (c). Data are presented as the average of three independent determinations ( ± standard deviation). The source data of the proanthocyanidin detrminations in (c) and (d) are provided in the Source Data file.

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