Table 3 Kinetic parameters for malonyl-CoA of THC production catalyzed by CHS of some land plants in the presence (+) and absence (–) of CHILa.

From: A conserved strategy of chalcone isomerase-like protein to rectify promiscuous chalcone synthase specificity

Enzyme

CHIL

Kineticsb

kcat (min−1)

K’ (µM)

kcat/K’ (min−1 μM−1)

Hill coeff.

PpCHS

+

S

31.5 (±0.6)

9.14 (±0.36)

3.45

2.96 (±0.32)

–

S

2.60 (±0.12)

3.23 (±0.22)

0.80

3.25 (±0.85)

SmCHS

+

S

23.5 (±0.4)

3.86 (±0.14)

6.09

2.11 (±0.14)

–

S

5.62 (±0.21)

1.15 (±0.09)

4.90

2.71 (±0.52)

GbCHS

+

S

28.9 (±1.7)

8.97 (±0.80)

3.22

2.46 (±0.45)

–

S

8.01 (±0.36)

3.60 (±0.22)

2.22

3.03 (±0.59)

OsCHS-1

+

S

7.03 (±0.36)

7.60 (±0.58)

0.93

2.31 (±0.34)

–

S

2.41 (±0.11)

3.35 (±0.16)

0.72

3.00 (±0.51)

AmCHS

+

S

35.4 (±1.4)

12.2 (±1.6)

2.90

 1.73 (±0.31)

–

S

8.47 (±0.14)

2.57 (±0.10)

3.30

3.77 (±0.73)

  1. a50 μM p-coumaroyl-CoA was used as the starter substrate.
  2. bS, sigmoidal. Sigmoidal v vs [S] plots were obtained and fit to the Hill’s equation. For details, see Methods.
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