Fig. 5: Most Pv parasites infecting individuals displaying antibodies highly inhibiting the PvDBP-red blood cell binding have multiple copies of pvdbp. | Nature Communications

Fig. 5: Most Pv parasites infecting individuals displaying antibodies highly inhibiting the PvDBP-red blood cell binding have multiple copies of pvdbp.

From: Amplification of Duffy binding protein-encoding gene allows Plasmodium vivax to evade host anti-DBP humoral immunity

Fig. 5

a Distribution of parasites with single pvdbp copy (n = 151, open bars) or multiple copies (n = 116, plain bars) across patients with binding inhibitory antibodies ranging from < 10% inhibition to > 90%. Individual plasma (at 1:5 dilution) are tested for their capacity to inhibit the binding of rDBPII (Sal1 allele) to red blood cells. The proportion of pvdbp-amplified isolates compared to single copy ones is significantly different only for patients displaying more than 90% binding inhibitory activity (7/116 and 1/151 respectively, Fisher’s exact test, P = 0.023). b Binding inhibitory antibodies from infected patients are strain transcending. The plasma (at 1:5 dilution) from all eight patients (plain circles infected with multiple pvdbp copies parasites and open circles with single copy) show >90% binding inhibition to three different DBPII alleles (Sal1, P and 7.18) similarly to plasma from malaria naïve individuals spiked with the humab 092096. Naïve plasma display <10% binding inhibition. Source data are provided as a Source Data file.

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