Fig. 4: Homotypic, heterosubtypic, and cross-group protection of mice immunized with H1 stem nanoparticle variants. | Nature Communications

Fig. 4: Homotypic, heterosubtypic, and cross-group protection of mice immunized with H1 stem nanoparticle variants.

From: Glycan repositioning of influenza hemagglutinin stem facilitates the elicitation of protective cross-group antibody responses

Fig. 4

BALB/c mice (N = 10) were immunized with 2 µg of H1ssF WT, H1ssF gN38 or H1ssF R38 on weeks 0, 4, and 8, and challenged between weeks 16 and 18 with 20 × LD50 of A/California/07/09 (H1N1) virus (a), 25 × LD50 of A/Vietnam/1203/04 (H5N1) virus (b), or 10 × LD50 of A/Anhui/1/13 (H7N9) virus (c). All viruses were given intranasal inoculation. Mice were monitored twice daily for their weight and activity for 14 days post infection. Survival curve (left) and associated weight loss curve (right) were plotted for each virus challenge (a–c). For weight loss curves, lines indicate group mean ± s.d. (shaded). Statistical test to compare multiple Kaplan–Meier curves was carried out by using Mantel-Cox log-rank test with Bonferroni correction. Displayed results are representative of two independent experiments (H1N1 and H5N1) or cumulative of two experiments (H7N9). Source data are provided as Source Data file.

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