Fig. 4: H59 forms a dynamic adaptor for Ebp1 binding. | Nature Communications

Fig. 4: H59 forms a dynamic adaptor for Ebp1 binding.

From: MetAP-like Ebp1 occupies the human ribosomal tunnel exit and recruits flexible rRNA expansion segments

Fig. 4

a The H59–Ebp1 interface. Atomic models of rRNA H59 (green) and Ebp1 helix α6 (blue) are superposed to the cryo-EM density (transparent gray). Overall orientation as in Fig. 3a and as indicated by the small representation in the corner. b Exemplary cryo-EM density of the Ebp1–ribosome interaction for the Ebp1 helix α6. c Structural details of the Ebp1–H59 interaction. Hydrogen bonds (dashed lines) and stacking interactions (parallel lines) are indicated. View is vertically rotated by 180° in respect to panels (a) and (b). d The Watson–Crick like readout of U2708. e Structural details of the Ebp1–H59–eL19 (brown) interaction around the bulged-out G2711 nucleotide. f H59 in the Ebp1-docked position rolled onto eL19 helix α3. In all panels, the cryo-EM density after 2-body multibody refinement is shown (transparent gray).

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