Fig. 5: A generalizable method for quantitative analysis with anti-Kasha-active chromophores. | Nature Communications

Fig. 5: A generalizable method for quantitative analysis with anti-Kasha-active chromophores.

From: De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules

Fig. 5

a Quantitative sensing platform. Chemical structures of DCM-IFC probes with different biomolecular-recognition groups: (b) acrylate group towards Cys; (f) 2,4-dinitrobenzenesulfonyl towards GSH; (j) disulfide bond towards GSH, sense-and-release. Fluorescence spectra (c, g, k), ratiometric response with internal reference signal (d, h, l, Source data are provided as a Source Data file.) and dual-channel linear ratio analysis (e, i, m, Source data are provided as a Source Data file.) of DCM-IFC-1, DCM-IFC-2, DCM-IFC-3, and DCM-IFC-4 for various analytes. Note: all fluorescence spectra were measured in a mixture solution of MeCN/phosphate-buffered saline (PBS, pH = 7.4), λex = 480 nm.

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