Fig. 2: JMJD1C promotes lipogenesis.
From: Histone demethylase JMJD1C is phosphorylated by mTOR to activate de novo lipogenesis
a Immunoblotting of lysates from HepG2 cells infected with JMJD1C adenovirus (left). RT-qPCR for mRNA levels (middle) and nascent RNA levels (right) of lipogenic genes in HepG2 cells with or without insulin treatment. n = 4 wells of cells per group. Experiment was repeated three times. b Immunoblotting of lysates from HepG2 cells infected with sh-JMJD1C adenovirus (left). RT-qPCR for mRNA levels of lipogenic genes (middle) and mRNA levels of lipogenic genes in HepG2 cells with or without 10 µM Methylstat treatment (right). n = 4 wells of cells per group. c Immunoblotting for JMJD1C protein and RT-qPCR for mRNA levels (left) in livers of mice 10 days after tail-vein injection of 2 × 108 pfu JMJD1C adenovirus in PBS (n = 5). Mice were fasted for 16 h and then refed with HCD for 8 h before tissue harvesting. mRNA levels of lipogenic genes (middle). Immunoblotting of lysates from livers for Fas and Srebp-1c (right). d De novo lipogenesis, e Oil red O staining of liver tissue sections (left), and liver triglyceride levels (right) n = 5 per group. f Jmjd1c protein and mRNA levels (left two) in livers of mice 10 days after tail-vein injection of sh-JMJD1C adenovirus (n = 5). mRNA levels of lipogenic genes (right). g De novo lipogenesis, h Oil red O staining of liver tissue sections (left) and liver triglyceride levels (right). Data are expressed as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, determined by two-tailed t-test. Scale bar = 100 μm. Source data are provided as a source data file.
