Fig. 3: Lipogenesis is impaired in liver-specific JMJD1C knockout mice.
From: Histone demethylase JMJD1C is phosphorylated by mTOR to activate de novo lipogenesis
a Schematics of conditional knockout allele of JMJD1C (left). RT-qPCR for Jmjd1c mRNA levels in tissues, n = 5 mice (middle) and immunoblotting of liver tissue lysates with JMJD1C antibody (right). b RT-qPCR for mRNA (left), immunoblotting of liver tissue lysates with Fas and Srebp-1c antibodies (middle), and nascent RNA (right). c Oil red O staining of liver tissue sections and liver triglyceride level. d Serum triglyceride and FFA levels in 12-week-old mice on chow diet. e Periodic acid–Schiff (PAS) staining for glycogen of liver tissues and glycogen levels in liver. a–e n = 8 mice per group. f–n JMJD1C-LKO mice and their WT littermates were fed a High CHO (70 kcal% carb) for 4 months after weaning, n = 6 mice per group. f mRNA (left) and nascent RNA (right), g de novo lipogenesis, h liver triglycerides, i Oil red O staining of liver tissue sections, j serum triglyceride levels, k liver glycogen levels, l and tissue weights. m GTT and ITT for high-CHO diet, and n serum insulin level. Data are expressed as means ± SEM. *P < 0.05, **P < 0.01, determined by two-tailed t-test. Scale bar = 100 μm. Source data are provided as a source data file.
