Fig. 1: Tdp2−/− Atm−/− causes the spontaneous accumulation of DSBs and etoposide hypersensitivity in mice. | Nature Communications

Fig. 1: Tdp2−/− Atm−/− causes the spontaneous accumulation of DSBs and etoposide hypersensitivity in mice.

From: Endogenous topoisomerase II-mediated DNA breaks drive thymic cancer predisposition linked to ATM deficiency

Fig. 1

a Endogenous DSB occurrence in Tdp2+/+ Atm+/+, Tdp2−/− Atm+/+, Tdp2+/+ Atm−/− and Tdp2−/− Atm−/− primary MEFs measured by 53BP1 foci formation. The percentage of cells with more than one focus (left) and representative images (right) of 53BP1 foci (green) and DAPI staining (blue) are shown for each genotype. MEFs isolated from three different embryos were analysed per condition. Mean ± SEM and statistical significance by one-way ANOVA with Bonferroni post-test is shown (F = 5,402). b Body weight of 8-week old mice cohorts intraperitoneally injected with a single dose of etoposide (25 mg/kg). Average ± SEM of the percentage of initial body weight from five mice and statistical significance by Two-way ANOVA with Bonferroni post-test is shown (F = 29,11). Tdp2+ indicates both Tdp2+/+ and Tdp2+/− genotypes. Representative images of Haematoxylin-Eosin stained jejunum slices obtained 6 days after etoposide exposure are shown.

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