Fig. 4: TOP2B colocalizes with cohesin at promoter, enhancer and insulator regions in thymocytes.

a Genome-wide distribution of 1003 TOP2B ChIPseq called peaks at different genomic features (promoters, enhancers, insulators and others) in wild-type primary thymocytes. Chromatin states were defined using ENCODE ccREs registry: H3K4me3, TSS and low H3K27ac for promoters, H3K27ac and low H3K4me3 for enhancers and high CTCF and no presence of the previous histone marks for insulator-like regions.TOP2B peak distribution compared to the entire genome (top), and global signal profile compared to IgG control (bottom) at each genomic feature is shown. TOP2B peaks were defined as common peaks between TOP2B ChIPseq experiments using two different antibodies. b Overlap of TOP2B and RAD21 peaks in wild-type thymocytes. c Genome browser view of TOP2B, RAD21, CTCF, POLR2A, H3K4me3 (ENCODE) and H3K27ac (ENCODE) signal tracks at a representative genomic region encompassing an active promoter (left, positive for POLR2A, H3K4me3 and H3K27ac) and an insulator (right,  negative for POLR2A, H3K4me3 and H3K27ac, and positive for CTCF and RAD21). Sites highlighted in grey show sites of major TOP2B signal accumulation over the region. d Heatmaps of TOP2B (this study), POLR2A (ENCODE), RAD21⁴⁷ and CTCF (ENCODE) ChIPseq signals at TOP2B peaks (± 5 kb). K-means clusters based on the different signals represented are shown divided by black lines and named as C1 and C2.