Fig. 6: TOP2B and cohesin colocalize with RAG genome-wide. | Nature Communications

Fig. 6: TOP2B and cohesin colocalize with RAG genome-wide.

From: Endogenous topoisomerase II-mediated DNA breaks drive thymic cancer predisposition linked to ATM deficiency

Fig. 6

a Heatmaps of RAG240, RAG140, TOP2B, RAD21 and ENDseq (DSBs) signal at RAG1 and RAG2 peaks (merged) in wild-type and Atm−/− primary thymocytes, as indicated. K-means clusters based on RAG1 and RAG2 signal are shown as C1 and C2. b Overlap of TOP2B and RAG peaks. Peaks of the RAG complex were defined as a merge of RAG1 and RAG2 proteins. c Global profile of TOP2B signal at RAG1 or RAG2 peaks. Control IgG signal is also shown. d Global profile of ENDseq signal at either RAG (merge of RAG1 and RAG2 peaks) or TOP2B-RAD21 (overlap between TOP2B and RAD21) peaks in wild-type, Atm−/− and Rag2−/− primary thymocytes.

Back to article page