Fig. 2: Neurons with distinct functional characteristics are sensitive to disruption of retinal horizontal direction selectivity in area RL.

a Response matrix composed of TF-dependent response amplitudes and DSI for RL L2/3 DS cells sorted by TF preference. b Two-dimensional (2D) visualization of the 1st and 2nd principal components for the response matrix shown in a. Each point represents one neuron. c TF preference of individual RL neurons. d Fraction of neurons in 8 × 8 grids (gray lines) calculated from the PCA plot shown in b. e Fraction difference map between control and Frmd7^tm mice. Black and white asterisks: significantly decreased and increased fractions in Frmd7^tm mice, respectively, P < 0.05, two-sided χ² test with Yates correction. f Peak response amplitude as a function of TF for three groups (decreased, increased, or unchanged in Frmd7^tm mice) in control (572, 304, and 575 DS cells, respectively) and Frmd7^tm mice (219, 508, and 659 DS cells, respectively). Error bars are SEM. g TF-dependent tuning characteristics of individual RL neurons from the three groups in control and Frmd7^tm mice. Angular coordinate: preferred direction. Radial coordinate: DSI. Inner circle: DSI of 0.5. h Response matrix composed of TF-dependent response amplitudes and DSI for PM L2/3 DS cells sorted by TF preference. i 2D visualization of the 1st and 2nd principal components for the response matrix shown in h. Each point represents one neuron. j TF preference of individual PM neurons. k Fraction of neurons in 8 × 8 grids (gray lines) calculated from the PCA plot in i for control and Frmd7^tm mice. l Fraction difference map between control and Frmd7^tm mice. m Peak response amplitude as a function of TF for the unchanged group in control (1098 DS cells) and Frmd7^tm mice (1217 DS cells). Error bars are SEM. n TF-dependent tuning characteristics of individual PM neurons from the unchanged group in control and Frmd7^tm mice. Angular coordinate: preferred direction. Radial coordinate: DSI. Inner circle: DSI of 0.5. Source data are provided as a Source Data file.