Fig. 4: V1 L2/3 neurons with distinct functional characteristics are sensitive to disruption of retinal horizontal direction selectivity.

a Response matrix composed of TF-dependent response amplitudes and DSI for all pooled V1 L2/3 DS cells (target-unspecific, PM-p, and RL-p) sorted by TF preference. b Two-dimensional (2D) visualization of the 1st and 2nd principal components for the response matrix shown in a. Each point represents one neuron. c TF preference of individual V1 neurons. d Fraction of neurons in 8 × 8 grids (gray lines) calculated from the PCA plot shown in b for control and Frmd7^tm mice. e Fraction difference map between control and Frmd7^tm mice. Black and white asterisks: significantly decreased and increased fractions in Frmd7^tm mice, respectively, P < 0.05, two-sided χ² test with Yates correction. f Peak response amplitude as a function of TF for three groups (decreased, increased, or unchanged in Frmd7^tm mice) in control (886, 845, and 591 DS cells, respectively) and Frmd7^tm mice (169, 825, and 381 DS cells, respectively). Error bars are SEM. g TF-dependent tuning characteristics of individual V1 neurons from the three groups in control and Frmd7^tm mice. Angular coordinate: preferred direction. Radial coordinate: DSI. Inner circle: DSI of 0.5. h Relationship between effect of Frmd7^tm mutation and enrichment in PM-p or RL-p neurons. x-axis: Index comparing axonal projection pattern for the individual grids in e that were decreased and increased in Frmd7^tm mice; groups with positive and negative index values are enriched in RL- and PM-projecting neurons, respectively. y-axis: Index comparing sensitivity to altered retinal direction selectivity for grids; grids with a positive and negative index value are decreased and increased in Frmd7^tm mice, respectively. Source data are provided as a Source Data file.