Fig. 7: Preference of retinal neurons to posterior motion at higher TFs is disrupted in Frmd7tm mice.
From: A segregated cortical stream for retinal direction selectivity
a Two-photon calcium imaging was performed on retinas from control (1157 cells; four mice) and Frmd7tm mice (953 cells; four mice). b Left: example control and Frmd7tm retinal neurons expressing GCaMP6s (scale bar, 10 µm). Right: trial-averaged fluorescence (ΔF/F0) time courses for the same. Shading indicates SEM. c Tuning curves for neurons shown in b. Error bars are SEM. Solid line: Gaussian fit. d Response amplitude as a function of motion direction (nasal [N], temporal [T], inferior [I], and superior [S]) and TF for retinal cells. White asterisk: significantly decreased response in Frmd7tm mice (two-sided Mann–Whitney U-test). e Fraction of horizontally- and vertically tuned retinal ON-OFF DS cells (425 and 342 ON-OFF DS cells in control and Frmd7tm mice, respectively) at 0.3 and 1.2 Hz (two-sided χ2 test with Yates correction). f Fractional distributions of preferred motion directions for ON-OFF DS cells at 0.3 and 1.2 Hz, normalized to the largest fraction across genetic groups. g Distribution of preferred motion directions at 0.3 and 1.2 Hz in ON-OFF DS cells (two-sided Kolmogorov–Smirnov test). h TF-dependent characteristics of ON-OFF DS cells. Angular coordinate: Directional preference at 1.2 Hz. Radial coordinate: Ratio of response amplitudes at 1.2 and 0.3 Hz. Inner circle: response ratio of 1. i Ratio of response amplitudes at 1.2 and 0.3 Hz as a function of motion direction preference of cells that showed ON-OFF DS responses (two-sided Mann–Whitney U-test). Center line is median, box limits are 25th and 75th percentiles, and whiskers show minimum and maximum values. j Schematic diagram of proposed neural pathway linking retinal ON-OFF DS cells to RL DS cells. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant. Source data are provided as a Source Data file.
