Fig. 6: Reliable image representation across trials.

a Examples of single-trial reconstructed images (top) and response patterns in a FOV (bottom). The first panel is a stimulus image, and the last panel is a trial-averaged image. FOV size: 507 micron each side. The color code for each dot indicates the response amplitude of each cell. b Single-trial evoked responses to the image in a. c Across-trial similarity of the reconstructed images (left) and the response patterns of responsive cells (right). The across-trial similarity was the Pearson’s correlation coefficient between a single-trial reconstructed image (or response pattern) and their trial average. d Across-trial variability of the reconstructed images (left) and the response patterns of responsive cells (right). The normalized squared error between a single-trial image (or response pattern) and their trial average was computed for the across-trial variability. e Reconstructed image from a set of overlapping cells (cell #1–9 in b). Upper left: stimulus image and trial-averaged reconstructed images from the nine overlapping cells. Cyan dotted line: the overlapping area. Upper right: representation (reverse filters) of the overlapping cells. Red line: the representation area. Cell #1 was the reference cell. Lower: single-trial representation patterns of three example cells (cell #1, 2, and 3) selected from the overlapping cells. Bottom: single-trial reconstructed images (upper) and single-trial response patterns in an FOV (lower) obtained from the nine overlapping cells. The brightness of each color dot in the lower panels indicates response amplitude of each cell. f Across-trial variability of a local part of reconstructed image (cyan dotted line in e) against the number of overlapping cells used for the reconstruction in the example case in e. Thick and thin lines are the median and 25th or 75th percentile, respectively (n = 200 random sequences of cell adding). g Across-trial variability against the percentage of overlapping cells used for the reconstruction. Black lines: raw data. Orange lines: trial-shuffled data. Thick and thin lines are median and 25th or 75th percentile, respectively. c, d, g N = 24 planes. The stimulus images in a and e are adapted from the database in ref. ⁵⁶ with permission. Source data are provided as a Source Data file.