Fig. 4: Confounding of intratumor clonal lymphocytes inference from barcode multiplets.
From: Inference and effects of barcode multiplets in droplet-based single-cell assays
a Schematic of intra-tumor lymphocytes identified from single-cell V(D)J sequencing on the 10× platform. b Identification of two presumed clonotypes composed of five and four barcodes. These clonotypes are likely to have been derived from one cell observed multiple times via barcode multiplets. c Example of a presumed clone composed of five barcodes with multiple constant sequences. d, e Overall summary of prevalence of d B-cell and e T-cell clone size before and after adjusting for observed rates of barcode multiplets in single-cell data. Error bars represent standard errors of the mean across n = 100 independent permutations from one experimental dataset per receptor sequence. Source data are available in the Source Data file.
