Fig. 4: Synergistic effect of 3H3 and antibiotic reduces S. Typhimurium biofilm. | Nature Communications

Fig. 4: Synergistic effect of 3H3 and antibiotic reduces S. Typhimurium biofilm.

From: Salmonella Typhimurium biofilm disruption by a human antibody that binds a pan-amyloid epitope on curli

Fig. 4

a S. Typhimurium biofilms were untreated or were incubated in the presence of 0.5 mg/ml control antibody 6A or 0.5 mg/ml 3H3. After 48 h, biofilms were subjected to 30 μg/ml ampicillin or not for an additional 24 h. Biofilms were stained with Syto9 (green), washed extensively, and visualized using Leica TCS confocal microscopy at ×63. Scale bars represent 25 μm. b Biofilm thickness (μm) was determined from z-stacks using Leica TCS software. Mean and SE were calculated from results from at least three independent experiments. *p < 0.05, **p < 0.01 as determined by Student’s t-test.

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