Fig. 6: Treatment with monoclonal antibody 3H3 enhances uptake of bacteria by macrophages.

a Wild-type bone marrow derived macrophages (BMDMs) were treated with 100 μl supernatants from biofilms grown in the absence (untreated) or presence of 0.5 mg/ml 3H3. After 1 h, gentamicin was added, cells were lysed, and bacteria were enumerated as colony forming units. b Wild-type BMDMs were treated with supernatants from biofilms grown in the absence (untreated) or presence of 0.5 mg/ml 3H3 adjusted to an optical density of 0.5 at 600. After 1 h, gentamicin was added, cells were lysed, and bacteria were enumerated as colony forming units. c 1 × 10⁶ wild-type immortalized macrophages were stimulated with 5 μg of untreated curli (CR Curli) or 3H3 incubated curli (3H3 CR Curli) for 1 h at 37 C and 5% CO_2. Cells were washed 3× with PBS to remove excess curli and macrophages were stained with DAPI (blue). Internalization of the curli (red) was imaged using a Leica TCS confocal microscopy at ×63. Scale bars represent 25 μm. Mean and SE were calculated from results from at least three independent experiments. *p < 0.05, **p < 0.01 as determined by Student’s t-test.