Fig. 6: Catalytic tetrad.
a, b DEDH tetrads of hAgo2 (PDB: 4W5N) a and Siwi (PDB: 5GUH) b. c Slicer activities of Siwi. The immunopurified FLAG-tagged wild-type Siwi and mutants were incubated with the internally 32P-labeled substrate RNA (piRNA-4 target), and the reaction products were analyzed by denaturing urea-PAGE. d DVDK tetrad of Piwi. e Sequences of the target RNA (flam target) and the piRNAs (flam-piRNA-1 and flam-piRNA-2). f Slicer activities of Piwi. The immunopurified FLAG-tagged wild-type Piwi or slicer-Piwi was incubated with the internally 32P-labeled substrate RNA (flam target), and the reaction products were analyzed by denaturing urea-PAGE. HSEH, the slicer-Piwi K617H/A625S/V653E/K818H mutant. Source data are provided as a Source Data file.
