Fig. 3: Copper uptake mediated by STEAP4 sustains NF-κB activation and inhibits apoptosis. | Nature Communications

Fig. 3: Copper uptake mediated by STEAP4 sustains NF-κB activation and inhibits apoptosis.

From: Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis

Fig. 3

a Western blot analysis of STEAP4-inducible Ls174t cells stimulated with IL-17 in the presence or absence of Dox and/or TTM. b Western blot analysis of wild-type (WT) and STEAP4 knockout (St4 KO) Ls174t cells stimulated with IL-17 for indicated hours in the presence or absence of 10μesCu(II). c Western blot analysis for nuclear translocation of p65 in the presence or absence of TTM under IL-17 treatment in WT and St4 KO Ls174t cells stimulated with IL-17 for indicated hours in the presence or absence of 10μabCu(II). d Densitometric quantification of (c). Error bar shows S.E.M. of quantification from three biologically independent cells. e, f Western blot analysis of primary colon organoids from wild-type (WT) or STEAP4 knockout (St4 KO) mice stimulated with IL-17 for indicated hours in the presence or absence of Cu(II). g Western blot analysis showed Steap4 overexpression inhibited apoptosis, which can be reversed by TTM. h 5-FU-induced cell apoptosis in Steap4 WT and KO organoids primed or unprimed with IL-17. Unless specified otherwise, data represent three independent experiments with similar results.

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