Fig. 2: Microscopy images of microcapsules with predominately lipid in the interior.
From: Primary cell wall inspired micro containers as a step towards a synthetic plant cell
CLSM images of a, b microcapsules exposed to rhodamine 6 G (Rh-6G, 0.01 mg mL−1) and c, d sulforhodamine 101 (SR-101, 0.5 mg mL−1). Insets show intensity line profiles obtained from the marked lines. a, c and b, d are fluorescence and transmission images, respectively. e The OA/oleate lipids were organized concentrically in the periphery (below the CNF/pectin capsule wall) of the microcapsules, observed as Maltese crosses in POM. In f, light microscopy image of empty and collapsed microcapsule walls (i.e., devoid of OA/oleate cores). The capsules were emptied by increasing the pH, see Supplementary Movie 1. The capsule walls were stained blue with calcofluor-white stain. The arrows point to burst cavities. All images are representative of three experiments. Scale bars: 20 µm a–d and f, and 50 µm e.
