Fig. 4: The nanostructure of the encasing CNF/pectin wall.
From: Primary cell wall inspired micro containers as a step towards a synthetic plant cell
CLSM transmission a and fluorescence b images of a plantosome stained with calcofluor-white (blue). TEM micrographs c–e of the remaining (dry) pectin/CNF shell after removal of lipids (OA, POPC, and POPE) from the interior of plantosomes/microcapsules. A burst cavity was created during the lipid removal. Images in d and e are high-resolution images of the shell in c. In f, fluorescence intensity profile for a plantosome exposed to FITC–dextran (\(\bar M_w\)= 4 kDa, 1 mg mL−1, green). The lipid phase contained Rh-DOPE (red). Images a–b and c–f are representative of two and three repeated experiments, respectively. Scale bars: 10 µm a, b, 5 µm c, 2 µm d, 1 µm e, and 10 µm f.
