Table 1 Cryo-EM data collection, model refinement and validation statistics.
FPR2-Gi1-scFV16-WKY (EMDB- EMD-20126) (PDB 6OMM) | |
|---|---|
Data collection and processing | |
Magnification | 130,000 |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | ~67 |
Defocus range (μm) | 0.6-2.5 |
Pixel size (Å) | 1.029 |
Symmetry imposed | None |
Initial particle images (no.) | 1,231,594 |
Final particle images (no.) | 203,133 |
Map resolution (Å) | 3.17 |
FSC threshold | 0.143 |
Map resolution range (Å) | 50-3.2 |
Refinement | |
Initial model used (PDB code) | 6DDE |
Model resolution (Å) | 3.17 |
FSC threshold | 0.143 |
Model resolution range (Å) | 50-3.2 |
Map sharpening B factor (Å2) | -117.3 |
Model composition | |
Non-hydrogen atoms | 9134 |
Protein residues | 1142 |
Ligands | 8 |
B factors (Å2) | |
Protein | 74.7 |
Ligand | 74.0 |
R.m.s. deviations | |
Bond lengths (Å) | 0.006 |
Bond angles (°) | 1.153 |
Validation | |
MolProbity score | 1.48 |
Clashscore | 4.74 |
Poor rotamers (%) | 0.0 |
Ramachandran plot | |
Favored (%) | 96.44 |
Allowed (%) | 3.56 |
Disallowed (%) | 0.0 |
