Fig. 3: Comparison of functional properties of LeuT_W8A and LeuT_WT.

a Time course of l-[³H]Ala uptake in proteoliposomes containing LeuT_WT (black circles) or LeuT_W8A (blue squares) or in control liposomes (gray circles). b Time course of l-[³H]Phe uptake in proteoliposomes containing LeuT_WT (black circles) or LeuT_W8A (blue squares) or in control liposomes (gray circles). Data in a, b are means ± S.E, n = 2, 3. c, d Saturation binding of l-[³H]Leu (c) or l-[³H]Phe (d) performed in 200 mM NaCl using a scintillation proximity assay (SPA) on purified LeuT_WT (black circles) or LeuT_W8A (blue squares) in detergent solution. Data were fitted to a one site-specific binding model and are shown as percentage of max binding for each construct. All SPA results are means ± S.E. from n = 3–5 independent experiments performed in triplicates. Source data are provided as a Source Data file.