Fig. 1: PPARγ interacts with the transcriptional corepressor ID2 motif.
From: A molecular switch regulating transcriptional repression and activation of PPARγ
a Fluorescence polarization coregulator interaction assay shows a robust interaction between PPARγ LBD with corepressor ID2 peptides from NCoR and SMRT as well as a TRAP220 coactivator peptide (n = 6). b Mammalian two-hybrid assay in HEK293T cells measuring the effect of T0070907 (10 μM) on the interaction between the Gal4-PPARγ LBD and the VP16-NCoR RID (n = 6; mean ± s.d.). c Luciferase transcriptional reporter assay measuring the ligand-dependent change in PPARγ transcription (n = 4; mean ± s.d.). d TR-FRET biochemical assay measuring the ligand-dependent change in the interaction of PPARγ LBD with SMRT ID2, NCoR ID2, and TRAP220 ID2 peptides (n = 3; mean ± s.e.m.). Source data are provided as a Source Data file.
