Fig. 4: Vascular functionality of primed vs. naïve VP. | Nature Communications

Fig. 4: Vascular functionality of primed vs. naïve VP.

From: Vascular progenitors generated from tankyrase inhibitor-regulated naïve diabetic human iPSC potentiate efficient revascularization of ischemic retina

Fig. 4

a Endothelial function. Shown are representative flow cytometry (left panel) and immunofluorescent Dil-acetylated-LDL (Dil-Ac-LDL) endothelial uptake assays (right panel); merged phase contrast/ Ac-Dil-LDL-labeled primed DVP vs. N-DVP cells; Scale Bar = 100 μm. DVP cells were generated from primed vs. naïve isogenic DhiPSC line E1CA2. b Expanded (non-diabetic) VP and N-VP and (diabetic) DVP and N-DVP were quantitated for senescent cells by β-galactosidase activity colorimetric assay. Shown are independent isogenic comparisons of both non-diabetic primed VP and N-VP (i.e., generated from H9 hESC, E5C3 CB-hiPSC, C1.2, C2 fibroblast-hiPSC lines) and diabetic DVP and N-DVP (i.e., generated from E1CA1, E1CA2, E1C1 fibroblast-DhiPSC lines). Each quantitation is an independent measurement of EGM2 cultures at indicated matched passages for each VP and N-VP type. ***p < 0.001 (multiple unpaired t-tests). c Quantification of vascular tube lengths formed from in vitro Matrigel tube assays from primed non-diabetic VP (E5C3) and isogenic primed DVP vs. N-DVP (E1CA2). The number (n) of total measurements of each of the three experimental groups from 3–5 independent experiments per group is labeled. *p < 0.05 (unpaired t-tests).

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