Fig. 2: Autophagy-deficient Drosophila photoreceptors form supernumerary synapses. | Nature Communications

Fig. 2: Autophagy-deficient Drosophila photoreceptors form supernumerary synapses.

From: Autophagy-dependent filopodial kinetics restrict synaptic partner choice during Drosophila brain wiring

Fig. 2

a–e’ Representative images of R1–R6 and R7 photoreceptor axon terminals with Brpshort-GFP marked active zones in wild-type (a, a’), atg7 mutant (b, b’), atg6 mutant (c, c’), atg18 mutant (d, d’), and atg6, GMR > Atg6 (e, e’). R7 axon terminals are shown from distal (top) to proximal (bottom) medulla. Relative thicknesses of medulla layers are shown in Control R7 terminals panel (a) along R7 axon terminals. Red boxes show supernumerary synapses in loss of autophagy at distal part of R7 axon terminals. Repeated five to ten times independently with similar results. f, g Number of Brp puncta per terminal in R1-R6 (f) and R7 (g) photoreceptors. n = 40 terminals per condition. Kruskal–Wallis and Dunn’s as post-hoc test; **p < 0.01, ***p < 0.001, ****p < 0.0001. Error bars denote mean ± SEM. h Number of Brp puncta in distinct medulla layers along R7 axon terminals (see Methods for the definition of medulla layers and a for relative thicknesses of medulla layers). n = 22 terminals for control, n = 30 terminals for atg7, n = 27 terminals for atg6. Kruskal–Wallis and Dunn’s as post-hoc test; *p < 0.05, ***p < 0.001, ****p < 0.0001. Error bars denote mean ± SEM. Source data are provided as a Source Data file.

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