Fig. 1: Design of MTBD constructs containing a disulfide bond within the coiled-coil.
From: Structural basis for two-way communication between dynein and microtubules
a, b Schematic drawings of the MTBD mutants stabilized either in high-affinity (pink) or low-affinity (cyan) states. The positions of the residues mutated to cysteine (S3097C, I3101C, V3222C) are shown schematically (a) or in the homology model of yeast MTBD using the mouse SRS (Seryl-tRNA synthetase) chimera (PDB code 3ERR) as a template (b). c–e The analysis of MT-binding affinity of (c) MTBD-WT, (d) MTBD-Low, and (e) MTBD-High by co-sedimentation assay. Source data are provided as a Source Data File. f The overlaid 1H 15N HSQC (heteronuclear single quantum) spectra of MTBD-WT (black), MTBD-Low (cyan), and MTBD-High (pink). Selected signals are enlarged in the boxes to the right side.
