Fig. 5: Identification of the low- and high-affinity interface by TCS (transferred cross-saturation) experiments.
From: Structural basis for two-way communication between dynein and microtubules
a, b Plots of the intensity reduction ratio of the amide resonances of (a) MTBD-High and (b) MTBD-Low in TCS experiments. The error bars are calculated based on signal-to-noise ratios. Residues showing an intensity reduction ratio greater than 0.15 for MTBD-Low or greater than 0.4 for MTBD-High are colored red. Signals with standard deviations greater than 0.1 were excluded. Although V3133 in H2, which is not exposed to the surface of MTBD, showed a significant intensity reduction in TCS experiments of MTBD-High, this reduction was presumably due to spin diffusion from H3 residues. Source data are provided as a Source Data File. c–e Mapping of the residues with significant intensity reduction in TCS experiments of (c) MTBD-High and (e) MTBD-Low. The overall orientation of MTBD with respect to MTs is shown in (d).
