Fig. 2: Enhanced mitochondrial fragmentation accelerates wound closure. | Nature Communications

Fig. 2: Enhanced mitochondrial fragmentation accelerates wound closure.

From: Wounding triggers MIRO-1 dependent mitochondrial fragmentation that accelerates epidermal wound closure through oxidative signaling

Fig. 2

a Left, representative confocal images of mitochondria in WT and mutants; right, representative images of F-actin ring assembly (Pcol-19-GFP::moesin(juIs352)) at 1 h.p.w. (hour post-wounding). N = 4 independent experiments. b Quantitation of actin ring diameter in wounded animals (WT, n = 49; drp-1, n = 61; fzo-1, n = 62; eat-3, n = 79 animals). Bars indicate mean ± SEM, normalized to WT. ****P < 0.0001 (versus WT), One-way ANOVA, Dunnett’s post test. c Representative images of mitochondria in fzo-1(tm1133) or tissue-specific rescued strains. d Quantitation of actin ring diameter (WT, n = 51; fzo-1, n = 35; fzo-1;Pfzo-1-fzo-1, n = 18; fzo-1;Pdpy-7-fzo-1, n = 28; fzo-1;Phsp-16.2-fzo-1, n = 23; fzo-1;Pmyo-3-fzo-1, n = 23 animals) at 1 h.p.w. Bars indicate mean ± SEM. ****P < 0.0001 (versus fzo-1), One-way ANOVA Dunnett’s test. Two-tailed unpaired t-test for fzo-1 and WT animals. e Left top, representative images of mitochondria morphology. Rotenone (100 μM), antimycin A (100 μM). and FCCP (10 μM). f Actin ring formation after wounding. N = 3 independent experiments (e, f). g Quantitation of actin ring diameter in WT and drug treated animals (Ctl, n = 31; Rotenone, n = 28; antimycin A, n = 33; FCCP 5 μM, n = 20; FCCP 10 μM, n = 16 animals) at 1 h.p.w. Bars indicate mean ± SEM. **P = 0.016, ****P < 0.0001 (versus ctl), One-way ANOVA Dunnett’s test. h A scheme of fzo-1::GFP(zju136) and GFP nanobody mediated degradation system (G-DEG). i Representative images of fzo-1::GFP expression in animals with or without G-DEG. N = 3 independent experiments. j Quantitation of actin ring diameter after tissue-specific knockdown of fzo-1 (WT, n = 25; Psur-5;G-DEG, n = 39; Psur-5;G-DEG;fzo-1, n = 24; Pcol-19;G-DEG, n = 21; Pcol-19;G-DEG;fzo-1, n = 18; Pmyo-3;G-DEG, n = 26; Pmyo-3;G-DEG;fzo-1, n = 35 animals). Bars indicate mean ± SEM. ns, P = 0.8508, ***P = 0.0004, ****P < 0.0001, Two-tailed unpaired t-test. Scale, 10 μm (a, c, e, f, and i) and 5 μm (zoom-in c, e, and i). Source data are provided as a Source Data file.

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