Fig. 5: Roles of auxin modulation in cluster root formation of white lupin.
From: The genome evolution and low-phosphorus adaptation in white lupin
a Quantification of IAA contents (ng per g of fresh weight, ng g−1 FW) in different types of white lupin roots. n = 3 plants. Effects of auxin polar transport inhibitors (b) and EDTA (c) on the formation of cluster roots in white lupin under P-sufficient (+P) or P-deficient (-P) conditions. n = 4 plants for (b) and n = 3 plants for (c). Error bars indicate s.e.m. P value was calculated using the unpaired two-sided Student’s t test. CHPAA, auxin influx inhibitor 3-chloro-4-hydroxyphenylacetic acid; NPA, auxin efflux inhibitor 1-naphtuylphthalamic acid; EDTA, metal chelator ethylenediaminetetraacetic acid. d Neighbor-joining phylogenetic tree of ABCG36 and ABCG37 genes from A. thaliana, P. vulgaris, and L. albus. e Expression profiles of LaABCG36a–d and LaABCG37a–c in roots under P-sufficient (+P) condition and normal roots (NRs) or different developmental stages of cluster roots under P deficiency (−P). PE, pre-emergent zone, 2–3 cm behind the root tip of first-order laterals; YCR, young cluster root; MCR, mature cluster root; OCR, old cluster root. The source data underlying Fig. 5a–c are provided as a Source Data file.
