Fig. 3: Point mutations affect different steps along the Cyt1Aa bioactivation cascade. | Nature Communications

Fig. 3: Point mutations affect different steps along the Cyt1Aa bioactivation cascade.

From: Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade

Fig. 3

For each row, different letters indicate significant differences between mutants. 1Length and 2width of crystals. 3Productivity was visually estimated based on the quantity and aspect of the crystal-spore suspension collected. 4SP50 corresponds to the concentration at which 50% of crystals solubilize after 1 h incubation at RT. See Supplementary Table 2 for corresponding statistics. 5Protoxin solubilized without DTT, releasing a disulfide-bridged dimer (except for C7S), and 6with DTT, releasing a protoxin monomer. 7Toxin monomer activated by proteinase K. 8Capacity of each toxin species to generate a ladder pattern characteristic of membrane-bound oligomers (MBO) on 6% SDS-PAGE. See corresponding gels in Supplementary Fig. 9. 9LC50 corresponds to the concentration lethal for 50% of cell population. See Supplementary Table 2 for corresponding statistics. Scale bar = 0.2 µm. Source data are provided as a Source Data file.

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