Fig. 5: α-melittin-NPs induce antigen-specific T cells and antibody responses.
From: Melittin-lipid nanoparticles target to lymph nodes and elicit a systemic anti-tumor immune response
a–c C57BL/6 mice (n = 4 per group) were treated as described above (Fig. 3a), on day 21, the lymphocytes isolated from the tumor-draining LNs were restimulated with DCs pulsed with B16F10 tumor lysates and were analyzed by flow cytometry with intracellular cytokine staining. Representative flow cytometry plots (a) and cumulative results (b, c) are shown. d, e The B16F10 tumor cells were incubated with 5% serum that was collected from treated mice and age-matched naïve mice (n = 4 per group). Subsequently, these cells were stained with a DyLight649-conjugated mouse IgG-specific secondary antibody and were analyzed by flow cytometry. Flow cytometry plots (d) and statistical percentages (e) are shown. Data are shown as the mean ± SEM. n.s. not significant, *P < 0.05, **P < 0.01 and ***P < 0.001, as analyzed by one-way ANOVA with Bonferroni’s post hoc test (b, c, e). Source data are provided as a Source Data file.
