Fig. 2: Metabolic profiles of Grasp55−/− mice.
From: Grasp55−/− mice display impaired fat absorption and resistance to high-fat diet-induced obesity
a, b Analyses of energy intake (a) and energy expenditure (b) were conducted in 12-week-old Grasp55+/+ and Grasp55−/− mice fed normal diet (ND) or high-fat diet (HFD). Energy expenditure differences between Grasp55+/+ and Grasp55−/− mice are plotted in (b inset). Data are shown as average values from a 2-day observation (n = 6). c, d Plasma leptin levels of 12-week-old Grasp55+/+ and Grasp55−/− mice that were fasted for 16 h and fed ND (n = 6). Plasma leptin levels normalized to body fat mass of mice are presented in d (n = 6). e, f Fasting plasma levels of insulin (e) and glucose (f) were measured in 12-week-old Grasp55+/+ and Grasp55−/− mice fasted for 16 h (n = 6). g An insulin tolerance test was performed in mice fasted for 4 h. The relative ratios of blood glucose levels to basal glucose levels were determined at the indicated time points following intraperitoneal administration of human insulin (0.75 U kg−1 body weight) in 12-week-old Grasp55+/+ and Grasp55−/− mice (n = 6). Data are shown as mean ± SEM. n.s.: not significant, *p < 0.05, **p < 0.01. All p values were calculated by unpaired two-tailed Student’s t tests. Source data are provided as a Source Data file.
