Fig. 2: Lymph-derived activated CD4 T cells enter LN parenchyma via the subcapsular sinus floor and express chemokine and homing receptors. | Nature Communications

Fig. 2: Lymph-derived activated CD4 T cells enter LN parenchyma via the subcapsular sinus floor and express chemokine and homing receptors.

From: Efficient homing of T cells via afferent lymphatics requires mechanical arrest and integrin-supported chemokine guidance

Fig. 2

a Representative FACS plots showing the forward and side scatter of naïve (left) and in vitro-activated (right) CD4+ T cells as well as the expression of CD62L and CD44. b Expression of E- and P-selectin and chemokine receptors on activated CD4+ T cells; control CCR7 staining in Ccr7−/− activated CD4+ T cells (blue line). c Expression of integrin subunits β1 (CD29), β2 (CD18), β7, and αv (CD51) on activated CD4+ T cells (red lines); gray shaded areas, isotype control. d Representative immuno-fluorescence microscopy of popliteal LN sections 20 and 90 min after i.l. injection of in vitro-activated CD4+ T cells and e 90 min after i.l. injection of in vivo-activated GFP-OTII cells (green); red, anti-IgD; blue, anti-Lyve-1; scale bars, 100 µm.

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