Fig. 4: Translocation through the SCS floor but not migration into the lymph node parenchyma occurs independent of chemokine receptors. | Nature Communications

Fig. 4: Translocation through the SCS floor but not migration into the lymph node parenchyma occurs independent of chemokine receptors.

From: Efficient homing of T cells via afferent lymphatics requires mechanical arrest and integrin-supported chemokine guidance

Fig. 4

a Ex vivo 2-photon imaging revealed different migration behavior of activated CD4+ T cells within the SCS (left, white tracks), directly after crossing the SCS floor (middle, green tracks), or within the LN parenchyma (right, red tracks). Movies start 15 min after i.l. injection of activated CD4+ cells into wild-type recipient mice. b, c Quantitative analysis of migration velocity and straightness of in vivo-activated OTII cells within the SCS or when crossing the SCS imaged ex vivo (b) or in vivo (c). Data are derived from five to seven movies from three independent experiments; Wilcoxon signed rank test *p < 0.05; **p < 0.01; ***p < 0.001. d Ex vivo time-lapse imaging of a popliteal LN after i.l. injection of activated PTX-treated CD4+ T cells (green), showing migration activity in the LN SCS. e Ex vivo time-lapse imaging of a popliteal LN after i.l. injection of in vivo-activated OTII-GFP cells (green) into a Prox1-mOrange2 recipient mouse showing simultaneous transmigration (tracks in green) of individual cells at the same localization. Prox1 (red) indicates SCS lymphatic endothelial cells. f, g Confocal images of the SCS showing adoptively transferred cells (white) within the SCS while crossing the SCS floor. Counterstained with antibodies as indicated; scale bars: a 50 µm; d, e 20 µm; f, g 15 µm.

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