Fig. 5: Selectins, PLVAP, and mannan-binding proteins do not contribute to homing of lymph-derived activated CD4 T cells.

Quantitative analysis of adoptively transferred cells in popliteal LNs 90 min after i.l. injection of a, b, d 1:1 mixtures of activated CD4+ T cell combinations as indicated; c activated CD4+ T cells into different recipients; a relative distribution as schematically depicted and migration distance of activated T cells treated with isotype or anti-L-selectin mAb; b absolute distribution and migration distance of activated T cells treated with isotype or anti-P- and E-selectin Ig fusion protein (parench parenchyma, sin. sys sinus system); c relative distribution and absolute cell counts of activated T cells injected into recipients treated with isotype or anti-PLVAP mAb; d absolute distribution and migration distance of activated T cells treated with PBS or Mannan. Data are derived from three experiments with a total of six mice (a), or from two experiments with a total of six (b, c) and five (d) mice; error bars, SD; red bars, median; b, d Wilcoxon signed rank test *p < 0.05; c Mann Whitney test.