Fig. 4: Cerebellum-enriched INPP5A is downregulated in SCA17 knock-in mice. | Nature Communications

Fig. 4: Cerebellum-enriched INPP5A is downregulated in SCA17 knock-in mice.

From: Cerebellum-enriched protein INPP5A contributes to selective neuropathology in mouse model of spinocerebellar ataxias type 17

Fig. 4

a Heatmaps of dysregulated genes hierarchically clustered from the cerebellum (CB), striatum (STR), and prefrontal cortex (PFC) of 3-month-old SCA17 KI and age-matched WT mice (n = 3–4 mice per group). Data were presented as log2 fold change with adjusted P value < 0.05. b Summary of the numbers of differentially expressed genes in the CB, STR, and PFC in KI mice. c Venn diagrams indicating the numbers of downregulated or upregulated genes in the CB, STR, and PFC. d Western blotting of INPP5A levels in different tissues from 3-month-old WT mice. Vinculin was used as a loading control. e Real-time PCR assay of Inpp5a mRNA levels in the CB, STR, and PFC from 3-month-old KI mice. The relative mRNA levels of Inpp5a were obtained by normalizing values to an internal control, GAPDH, and analyzed with Student’s t test, t = 5.493, **P = 0.0054, n = 3 mice per group. f, g Western blotting (f) and immunofluorescent staining (g) of INPP5A in the cerebellum from 3-month-old WT and KI mice. Scale bar = 100 μm. In f, densitometric ratios of NeuN to β-actin were normalized to WT and analyzed with Student’s t test, t = 6.903, **P = 0.0023, n = 3 mice per group. Data are represented as mean ± SEM. Source data and full blots are provided as a Source Data file.

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