Fig. 6: Effect of C₁₂-G⁺, C₁₈-NMe₃⁺, and C₁₈-SMe₂⁺ on the rice blast fungus M. oryzae and the corn smut fungus U. maydis.

a Effect of treatment of M. oryzae conidia with the solvent (control), C₁₂-G⁺, C₁₈-SMe₂⁺, and C₁₈-NMe₃⁺. In control experiments, appressoria are formed (Control, open arrowheads), whereas all MALCs inhibit germination of M. oryzae conidia at high concentrations. Scale bar = 20 µm. b Effect of C₁₂-G⁺, C₁₈-NMe₃⁺, and C₁₈-SMe₂⁺ on germination of M. oryzae conidia. See Table 1 for EC₅₀ values. c Effect of C₁₂-G⁺, C₁₈-SMe₂⁺, and C₁₈-NMe₃⁺ on mitochondrial potential in M. oryzae. Values are background-corrected TMRM fluorescence intensity values. See also Supplementary Fig. 7b. d Effect of C₁₂-G⁺, C₁₈-SMe₂⁺, and C₁₈-NMe₃⁺ on mitochondrial potential in U. maydis. Values are background-corrected TMRM fluorescence intensity values. See also Supplementary Fig. 7d. e Effect of C₁₂-G⁺, C₁₈-SMe₂⁺, and C₁₈-NMe₃⁺ on the mROS levels in M. oryzae. Only C₁₈-SMe₂⁺ induces significantly mROS levels. f Effect of C₁₂-G⁺, C₁₈-SMe₂⁺, and C₁₈-NMe₃⁺ on the mROS levels in U. maydis. Only C₁₈-SMe₂⁺ induces significantly mROS levels. See also Supplementary Fig. 7e. Values (b) are shown as mean ± SEM, with sample size n = 3. Non-linear regression curves were calculated as dose-response inhibition (four parameters) in Prism5. c–f Whiskers’ plots, with 25th/75th percentiles (blue lines), medians (red line), and minimum and maximum (whiskers ends). Green lines indicate level of control median. Sample size n is indicated in each panel. Non-parametric Mann–Whitney testing (a, c, d); n.s. non-significant difference to control (P > 0.05) (d), ***P < 0.0001 (a, c, d). Student’s t-testing with Welch correction (f, h); n.s. non-significance (f, h) and ***P = 0.0002 (f, h). All P-values are two-tailed. See Supplementary Table 7 for experimental conditions. All source data are provided as a Source Data file.