Fig. 3: Evolutionary conservation of cell types between human and mouse predicts that VENs project sub-cortically. | Nature Communications

Fig. 3: Evolutionary conservation of cell types between human and mouse predicts that VENs project sub-cortically.

From: Transcriptomic evidence that von Economo neurons are regionally specialized extratelencephalic-projecting excitatory neurons

Fig. 3

a, b Excitatory neurons in human FI (red), human MTG (green), mouse ALM (cyan), and mouse VISp (purple) were integrated and aligned using Seurat v341 with default parameters, and visualized using UMAP. a Cells from each data-set co-cluster, indicating good matching of types between brain regions and species. b Eight Seurat clusters were identified using the Louvain algorithm and labeled based on expected cortical layer and projection target (as described in c). c Membership of cells from excitatory clusters in each data set in the Seurat clusters. Colors indicate the fraction of total cells per cluster assigned to each Seurat cluster (rows sum to 1). Data set clusters are grouped based on maximal fraction of cells in the cluster. Cortical layer of each cluster are inferred based on predominant cortical layer of cells from mouse and human data sets, with the exception of the layer 4 cluster (L4* IT) which primarily includes cells from layer 5 in structures without a layer 4. Projection targets of clusters are inferred based on known projection targets of clusters in mouse ALM and VISp (IT—intratelencephalic, ET—extratelencephalic, NP—near-projecting, CT—corticothalamic). The box highlights that Exc FEZF2 GABRQ is part of the L5 ET cluster.

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