Fig. 5: Distinctive electrophysiological properties of putative L5 VENs in ex vivo insula brain slices from a human neurosurgery patient. | Nature Communications

Fig. 5: Distinctive electrophysiological properties of putative L5 VENs in ex vivo insula brain slices from a human neurosurgery patient.

From: Transcriptomic evidence that von Economo neurons are regionally specialized extratelencephalic-projecting excitatory neurons

Fig. 5

a MRI scan indicating the location of the excised insula tissue specimen for research. b Best matched location in the Allen 2D coronal human brain reference atlas, with crosshairs centered on the short insular gyrus. Scale bar: 1 cm. c Biocytin-filled putative VEN in L5 of an ex vivo insula brain slice. Low magnification brightfield and DAPI image confirms the L5 location of the neuron. The boxed region bounding the biocytin-filled neuron is expanded at right. Inset: image of Alexa dye fill following patch clamp recording in live tissue. Scale bars: 1 mm and 100 µm. d Example traces of action potential firing pattern in response to current injection steps for a representative pyramidal neuron (PN) and VEN. Scale bars: 50 pA, 500 msec. e Summary plot of action potential firing in response to current injection steps. *p < 0.0001, 2-way ANOVA. f Summary plot of coefficient of variation (CV) for VENs vs. PNs. *p < 0.05, Mann–Whitney. g Summary plot of spike frequency adaptation (SFA) for VENs vs. PNs. *p < 0.05, Mann–Whitney. Center lines show mean and error bars show standard error of the mean.

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