Fig. 6: Hyperactivation of mTOR-related metabolic pathways in Terc−/− mice.
From: The mTOR pathway is necessary for survival of mice with short telomeres
Gene expression data obtained by RNAseq of three independent liver samples from Terc+/+ and G2 Terc−/− male mice subjected to control or rapamycin diet and sacrificed after 2 months of treatment were analyzed by Gene Set Enrichment Analysis (GSEA) to determine significantly enriched gene sets. a Venn diagram showing the overlapping pathways found significantly upregulated in control fed G2 Terc−/− versus Terc+/+ and those found significantly downregulated in rapamycin-treated G2 Terc−/− versus control fed G2 Terc−/− and in rapamycin-treated wild type versus control-fed wild type. Twenty-two common pathways were detected at the intersection of the three comparisons. Several pathways related to the mTOR pathway are listed in the box. b–j GSEA plots for the indicated pathways in liver samples. b–d G2 Terc−/− versus Terc+/+ control-fed mice. (e-g) G2 Terc−/− rapamycin-fed versus G2 Terc−/− control-fed mice. h–j Terc+/+ rapamycin-fed versus Terc+/+ control-fed mice. The red to blue horizontal bar represents the ranked list. Genes located at the central area of the bar show small differences in gene expression between the pairwise compared. At the red edge of the bar are located genes showing higher expression levels and at the blue edge of the bar are located genes showing lower expression levels. Red and blue arrows indicated upregulation and downregulation, respectively, of the pathway in the pairwise comparisons. Samples correspond to livers of three independent G2 Terc−/− or Terc+/+ male mice sacrificed after 2 months of control or rapamycin feeding. False discovery rates (FDR) are indicated.
