Fig. 1: EMC4, EMC6, and EMC7 promote SV40 infection. | Nature Communications

Fig. 1: EMC4, EMC6, and EMC7 promote SV40 infection.

From: Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry

Fig. 1

a–c CV-1 cells were transfected with scrambled or the indicated siRNA, and then with the indicated FLAG-tagged constructs. Cells were then infected with SV40 (MOI ~ 0.5), fixed, and stained with FLAG and large T-antigen antibodies. The percentages of T-antigen-positive cells were determined only in FLAG-expressing cells by using epifluorescence widefield microscopy. Values represent means ± SD from three independent experiments. D-E. CV-1 (d) and HEK 293 T (e) cells were transfected with the indicated siRNA, and the resulting cell extract was subjected to SDS-PAGE followed by immunoblotting using the indicated antibodies. The indicated EMC band intensities under the different knockdown conditions were quantified in the bottom panel. Values represent means ± SD from three independent experiments. Source data are provided as a Source Data file.

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