Fig. 6: EMC4 and EMC7 mediate ER-LE contact. | Nature Communications

Fig. 6: EMC4 and EMC7 mediate ER-LE contact.

From: Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry

Fig. 6

a Graphical representation of the split-GFP assay for detecting ER-LE contacts. b CV-1 cells transfected with the indicated construct(s) were fixed, stained with B14 and FLAG antibody, and analyzed under confocal microscope for GFP signal. This experiment was independently repeated three times. Scale bar = 10 µm. c CV-1 cells co-expressing GFP11-STARD3-FLAG and GFP (1-10)-B14 and transfected with either scrambled or EMC4+EMC7 siRNAs were fixed, stained with B14 and FLAG antibody, and analyzed under confocal microscope for GFP signal. Scale bar = 10 µm. d Mean GFP signal intensity from c was normalized with FLAG signal and quantified by FIJI/ImageJ. The image analysis was done on a single z-plane. Values represent means ± SD from three independent experiments. Unpaired Student two-tailed t-test was used to determine statistical significance. Source data are provided as a Source Data file.

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