Fig. 7: Specific domains of EMC4 and EMC7 mediate Rab7-binding essential for SV40 ER-arrival and infection.

a Diagram of wild-type and mutant EMC4 and EMC7. b EMC4 (lane 1-2) or EMC7 (lane 3–6) siRNA-treated HEK 293 T cells transfected with the indicated plasmid were lysed, the resulting extract incubated with FLAG antibody-conjugated agarose beads, and the precipitated material subjected to SDS-PAGE and immunoblotting. This experiment was independently repeated three times. c CV-1 cells were transfected with scrambled or the indicated siRNAs prior to transfection with the indicated FLAG-tagged constructs. Cells were then infected with SV40 (MOI ~ 0.5), fixed, and stained with FLAG and large T-antigen antibodies. The percentages of T-antigen-positive cells were determined in only FLAG-expressing cells by using epifluorescence widefield microscopy. Values represent means ± SD from three independent experiments. d CV-1 cells were transfected with scrambled or the indicated siRNAs prior to transfection with the indicated FLAG-tagged constructs. Cells were then infected with SV40 (MOI ~ 2), fixed, and stained with FLAG and VP2/VP3 antibodies. The mean VP2/VP3 signal intensity was determined by FIJI/ImageJ software in only FLAG-expressing cells by using epifluorescence widefield microscopy. Values represent means ± SD from three independent experiments. e CV-1 cells were transfected with the indicated EGFP-tagged construct. Cells were then infected with SV40, fixed, and stained with VP2/VP3 antibodies. The mean VP2/VP3 signal intensity was determined by FIJI/ImageJ software in only EGFP-expressing cells by using epifluorescence widefield microscopy. Values represent means ± SD from three independent experiments. Source data are provided as a Source Data file.